VEGF 165 (Active) 蛋白
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- 抗原
- VEGF 165
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- 原理
- VEGF 165 (human) (rec.)
- 交叉反应
- 人
- 产品特性
- Human VEGF 165 (165aa).
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.1ng/μg protein.
- Biological Activity Comment
- The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.
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VEGF 165 protein
宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) protein (His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
VEGF 165 (AA 207-371) protein (His tag)宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
VEGF 165 (AA 1-191) protein (His tag)宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
VEGF 165 protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 50 mM acetic acid to a concentration not lower than 50 μg/mL.
- 注意事项
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.
- 有效期
- 6 months
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- 抗原
- VEGF 165
- 背景
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Vascular Endothelial Growth Factor, VEGF-A, VPF
Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.
- 分子量
- ~38.2kDa
- UniProt
- P15692-4
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