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VEGF 165 (Active) 蛋白

This Recombinant protein is expressed in 大肠杆菌（E. Coli）.

产品编号 ABIN2746009

发货至: 中国

Quick Overview for VEGF 165 (Active) 蛋白 (ABIN2746009)

抗原

VEGF 165

蛋白类型

Recombinant

生物活性

Active

宿主

人

资源

大肠杆菌（E. Coli）

应用范围

SDS-PAGE (SDS)

纯度

>95 % (SDS-PAGE)

原理

VEGF 165 (human) (rec.)

交叉反应

人

产品特性

Human VEGF 165 (165aa).

内毒素水平

<0.1ng/μg protein.

Biological Activity Comment

The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.
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VEGF 165 (AA 27-191) protein
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

VEGF 165 (AA 27-191) protein (His-Avi Tag,Biotin)
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)
宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active

VEGF 165 (AA 27-191) protein (His-Avi Tag)
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

VEGF 165 (Homodimer) (Active) protein
宿主: 人宿主: Insect Cells Recombinant > 95 % by SDS-PAGE Active

VEGF 165 (AA 207-371) protein (His tag)
宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS

VEGF 165 (AA 27-191) protein (His tag)
宿主: 人宿主: Baculovirus infected Insect Cells Recombinant > 85% by SDS-PAGE SDS

VEGF 165 protein
宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS

VEGF 165 (Homodimer) (Active) protein
宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE Active

VEGF 165 (AA 27-190) (Active) protein
宿主: 大鼠宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute with 50 mM acetic acid to a concentration not lower than 50 μg/mL.

注意事项

After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.

储存条件

4 °C,-20 °C

储存方法

Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

有效期

6 months
抗原

VEGF 165

背景

Vascular Endothelial Growth Factor, VEGF-A, VPF
Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.

分子量

~38.2kDa

UniProt

P15692-4

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