EnhancedFasL protein (DYKDDDDK Tag)
This Recombinant protein is expressed in HEK-293 Cells.
Quick Overview for EnhancedFasL protein (DYKDDDDK Tag) (ABIN2745658)
抗原
EnhancedFasL
蛋白类型
Recombinant
宿主
人, 小鼠
资源
HEK-293 Cells
应用范围
Biochemical Assay (BCA), SDS-PAGE (SDS)
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标记
- DYKDDDDK Tag
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特异性
- Binds to human and mouse Fas (CD95, APO-1).
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交叉反应
- 人, 小鼠
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组件
- 1 Set: 1x10μg of FasL, Soluble (human) (rec.) 4x50μg of TNF Ligands Enhancer
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Source/Host: Produced in HEK 293 cells. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human FasL (aa 103-281). Concentration: FasL: 0.1mg/ml after reconstitution TNF Ligands Enhancer: 1mg/ml after reconstitution Purity: >95% (SDS-PAGE)Formulation: Lyophilized. Contains PBS. Endotoxin Content: FasL: <0.05EU/μg purified protein (LAL test, Lonza). TNF Ligands Enhancer: <0.01EU/μg purified protein (LAL test, Lonza). Reconstitution: FasL: reconstitute with 100μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. TNF Ligands Enhancer: reconstitute each vial with 50μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
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限制
- 仅限研究用
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浓度
- Lot specific
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. -
有效期
- 6 months
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- EnhancedFasL
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背景
- FasL, Soluble (human) (rec.) induces apoptosis in a concentration range of <1 ng/mL in the presence of 0.1 to 1 μg/mL of TNF Ligands Enhancer . (Optimal conditions must be determined individually for each cell line tested)
抗原
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