EnhancedFasL protein (DYKDDDDK Tag)
宿主: 人, 小鼠
宿主: HEK-293 Cells
Recombinant
BCA, SDS
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- 抗原
- EnhancedFasL
- 蛋白类型
- Recombinant
- 宿主
- 人, 小鼠
- 资源
- HEK-293 Cells
- 标记
- DYKDDDDK Tag
- 应用范围
- Biochemical Assay (BCA), SDS-PAGE (SDS)
- 特异性
- Binds to human and mouse Fas (CD95, APO-1).
- 交叉反应
- 人, 小鼠
- 组件
- 1 Set: 1x10μg of FasL, Soluble (human) (rec.) 4x50μg of TNF Ligands Enhancer
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Source/Host: Produced in HEK 293 cells. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human FasL (aa 103-281). Concentration: FasL: 0.1mg/ml after reconstitution TNF Ligands Enhancer: 1mg/ml after reconstitution Purity: >95% (SDS-PAGE)Formulation: Lyophilized. Contains PBS. Endotoxin Content: FasL: <0.05EU/μg purified protein (LAL test, Lonza). TNF Ligands Enhancer: <0.01EU/μg purified protein (LAL test, Lonza). Reconstitution: FasL: reconstitute with 100μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. TNF Ligands Enhancer: reconstitute each vial with 50μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
- 限制
- 仅限研究用
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- 浓度
- Lot specific
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - 有效期
- 6 months
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- 抗原
- EnhancedFasL
- 背景
- FasL, Soluble (human) (rec.) induces apoptosis in a concentration range of <1 ng/mL in the presence of 0.1 to 1 μg/mL of TNF Ligands Enhancer . (Optimal conditions must be determined individually for each cell line tested)
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