LTA Protein (Transcript Variant 2)
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北京 101111
Quick Overview for LTA Protein (Transcript Variant 2) (ABIN2733938)
抗原
See all LTA 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 2
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human TNF-beta / Lymphotoxin-alpha (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.
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Lymphotoxin-alpha (LTA) (Active) protein
LTA 宿主: 化学剂 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Lymphotoxin-alpha (LTA) (AA 58-205) protein (His-DYKDDDDK-Strep II Tag)LTA 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Lymphotoxin-alpha (LTA) (AA 35-205) protein (His tag)LTA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Lymphotoxin-alpha (LTA) (AA 34-202) protein (His tag)LTA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Lymphotoxin-alpha (LTA) (AA 1-205) protein (GST tag)LTA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Lymphotoxin-alpha (LTA) (AA 35-205) (Active) protein (His tag)LTA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, AcA Active
Lymphotoxin-alpha (LTA) (AA 35-205) (Active) protein (His tag)LTA 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS, AcA Active
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- LTA (Lymphotoxin-alpha (LTA))
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别名
- Tnf-Beta,lymphotoxin-alpha
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物质类
- Chemical
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背景
- Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. [UniProtKB/Swiss-Prot Function]
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分子量
- 18.6 kDa
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NCBI登录号
- NP_000586
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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