Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human Placenta growth factor / PGF (transcript variant 1) protein expressed in HEK293.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % , as determined by Coomassie stained SDS-PAGE.
内毒素水平
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 1-3 ng/ml, corresponding to a specific activity of 0.33 - 1 x 106 units/mg, determined by the dose dependent binding to VEGFR1-Fc chimera using a functional ELISA.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
5 mM citric acid, 0.15 M NaCl, 5 mM NaHPO4, pH 4.0
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011].