NAGPA Protein (Myc-DYKDDDDK Tag)
This Recombinant NAGPA protein is expressed in HEK-293 Cells.
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产品编号 ABIN2726733
发货至:
中国
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Quick Overview for NAGPA Protein (Myc-DYKDDDDK Tag) (ABIN2726733)
抗原
See all NAGPA 蛋白
NAGPA
(N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
应用范围
Antibody Production (AbP), Standard (STD)
纯度
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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标记
- This NAGPA protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human NAGPA protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA) (AA 309-408) protein (GST tag)
NAGPA 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))
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背景
- Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients.
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分子量
- 53.3 kDa
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NCBI登录号
- NP_057340
抗原
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