Isocitrate Dehydrogenase Protein (IDH) (DYKDDDDK Tag)
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Quick Overview for Isocitrate Dehydrogenase Protein (IDH) (DYKDDDDK Tag) (ABIN2723847)
抗原
See all Isocitrate Dehydrogenase (IDH) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Isocitrate Dehydrogenase protein is labelled with DYKDDDDK Tag.
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产品特性
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- Recombinant human Isocitrate dehydrogenase / IDH (C-term DDK tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
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Isocitrate Dehydrogenase (IDH) (Active) protein (DYKDDDDK Tag)
IDH 宿主: 人 宿主: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
Isocitrate Dehydrogenase (IDH) (Active) protein (His tag)IDH 宿主: 人 宿主: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
Isocitrate Dehydrogenase (IDH) (Active) protein (Myc-DYKDDDDK Tag)IDH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
Isocitrate Dehydrogenase (IDH) protein (Myc-DYKDDDDK Tag)IDH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Isocitrate Dehydrogenase (IDH)
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别名
- Isocitrate Dehydrogenase,idh
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背景
- Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
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分子量
- 47 kDa
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NCBI登录号
- NP_005887
抗原
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