产品编号 ABIN2723689
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for IL27 蛋白 (ABIN2723689)
抗原
IL27
(Interleukin-27 Subunit alpha (IL27))
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
HEK-293 Cells
应用范围
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
纯度
Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Interleukin-27 / IL27A protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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浓度
- > 50 μg/mL
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缓冲液
- 20 mM NaH2PO4, pH 6, 0.60 M NaCl
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL27 (Interleukin-27 Subunit alpha (IL27))
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别名
- Interleukin-27,il27a
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分子量
- 49 kDa
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NCBI登录号
- NP_663611
抗原
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