IL12A Protein (Transcript Variant 2)
This Recombinant IL12A protein is expressed in CHO Cells.
Quick Overview for IL12A Protein (Transcript Variant 2) (ABIN2723598)
抗原
See all IL12A 蛋白
IL12A
(Interleukin 12 alpha (IL12A))
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
CHO Cells
应用范围
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
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产品特性
- Transcript Variant 2
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Interleukin-12 alpha / IL12A (transcript variant 2) protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Assay #1: ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is > 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg. Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is
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Interleukin 12 alpha (IL12A) (AA 144-253) protein (GST tag)
IL12A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL12A (Interleukin 12 alpha (IL12A))
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别名
- Interleukin-12 Alpha,il12a
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分子量
- 75 kDa
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NCBI登录号
- NP_032377
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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