Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human Insulin-like growth factor II / IGF2 (transcript variant 2) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
内毒素水平
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 10 - 30 ng/ml, corresponding to a specific activity of 0.3 - 1 x 10^5 units/mg, determined by the dose dependent stimulation of MCF-7 cell proliferation.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
1 x PBS
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.