GREM1 Protein (Transcript Variant 1)
Quick Overview for GREM1 Protein (Transcript Variant 1) (ABIN2722232)
抗原
See all GREM1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 1
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human Gremlin-1 / GREM1 (transcript variant 1) protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11ug/mL.
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Gremlin 1 (GREM1) (Active) protein
GREM1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） WB, SDS Active
Gremlin 1 (GREM1) (AA 25-184) (Active) protein (Fc Tag)GREM1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Gremlin 1 (GREM1) (AA 25-184) protein (His-IF2DI Tag)GREM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Gremlin 1 (GREM1) (AA 25-124) protein (GST tag)GREM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Gremlin 1 (GREM1) (AA 1-184) protein (GST tag)GREM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Gremlin 1 (GREM1) (AA 25-184) (Active) protein (His tag)GREM1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- GREM1 (Gremlin 1 (GREM1))
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别名
- Gremlin-1,grem1
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背景
- This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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分子量
- 18.3 kDa
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NCBI登录号
- NP_037504
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途径
- Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location
抗原
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