G-CSF Protein (Transcript Variant 1)
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北京 101111
Quick Overview for G-CSF Protein (Transcript Variant 1) (ABIN2721513)
抗原
See all G-CSF (CSF3) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Transcript Variant 1
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human G-CSF (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than or equal to 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg.
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Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) protein
CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-208) (Active) proteinCSF3 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) protein (His tag)CSF3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 22-214) protein (His-IF2DI Tag)CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) protein (GST tag)CSF3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, AcA Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- G-Csf
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背景
- The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.
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分子量
- 18.7 kDa
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NCBI登录号
- NP_000750
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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