Dihydrofolate Reductase Protein (DHFR) (Myc-DYKDDDDK Tag)
Quick Overview for Dihydrofolate Reductase Protein (DHFR) (Myc-DYKDDDDK Tag) (ABIN2719443)
抗原
See all Dihydrofolate Reductase (DHFR) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Dihydrofolate Reductase protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human DHFR / DHFRP1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Dihydrofolate Reductase (DHFR) (AA 1-187) protein (His tag)
DHFR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Dihydrofolate Reductase (DHFR) (AA 1-187) protein (His tag)DHFR 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Dihydrofolate Reductase (DHFR) (AA 1-187) protein (GST tag)DHFR 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Dihydrofolate Reductase (DHFR) (AA 2-187) protein (His-SUMO Tag)DHFR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Dihydrofolate Reductase (DHFR)
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别名
- Dhfr,dhfrp1
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背景
- Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.
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分子量
- 21.3 kDa
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NCBI登录号
- NP_000782
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途径
- Mitotic G1-G1/S Phases
抗原
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