Bystin Protein (BYST) (Myc-DYKDDDDK Tag)
This Recombinant Bystin protein is expressed in HEK-293 Cells.
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Quick Overview for Bystin Protein (BYST) (Myc-DYKDDDDK Tag) (ABIN2715365)
抗原
Bystin (BYST)
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
应用范围
Antibody Production (AbP), Standard (STD)
纯度
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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标记
- This Bystin protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human Bystin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Bystin (BYST)
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别名
- Bystin
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背景
- Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.
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分子量
- 49.4 kDa
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NCBI登录号
- NP_004044
抗原
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