BMP4 Protein (Transcript Variant 1)
This Recombinant BMP4 protein is expressed in 大肠杆菌（E. Coli）.
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产品编号 ABIN2715301
发货至:
中国
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Quick Overview for BMP4 Protein (Transcript Variant 1) (ABIN2715301)
抗原
See all BMP4 蛋白
BMP4
(Bone Morphogenetic Protein 4 (BMP4))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
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产品特性
- Transcript Variant 1
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特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
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产品特性
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- Recombinant human BMP4 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml.
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Bone Morphogenetic Protein 4 (BMP4) (AA 303-408) protein (His-IF2DI Tag)
BMP4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Bone Morphogenetic Protein 4 (BMP4) (AA 293-408) proteinBMP4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
限制
- 仅限研究用
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缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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注意事项
- Resuspend the protein in the desired concentration in proper buffer
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
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别名
- Bmp4
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背景
- This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers.
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分子量
- 12 kDa
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NCBI登录号
- NP_001193
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途径
- Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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