alpha 2 Macroglobulin Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all alpha 2 Macroglobulin (A2M) 蛋白
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This alpha 2 Macroglobulin protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human Alpha-2-macroglobulin / A2M protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product A2M 蛋白
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- 别名
- alpha-2-Macroglobulin (A2M 产品)
- 背景
- Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [UniProtKB/Swiss-Prot Function]
- 分子量
- 160.8 kDa
- NCBI登录号
- NP_000005
- 途径
- Lipid Metabolism
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