alpha 2 Macroglobulin Protein (Myc-DYKDDDDK Tag)
This Recombinant alpha 2 Macroglobulin protein is expressed in HEK-293 Cells.
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Quick Overview for alpha 2 Macroglobulin Protein (Myc-DYKDDDDK Tag) (ABIN2714610)
抗原
See all alpha 2 Macroglobulin (A2M) 蛋白
alpha 2 Macroglobulin (A2M)
(alpha-2-Macroglobulin (A2M))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
应用范围
Antibody Production (AbP), Standard (STD)
纯度
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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标记
- This alpha 2 Macroglobulin protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human Alpha-2-macroglobulin / A2M protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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alpha-2-Macroglobulin (A2M) (AA 641-730) protein (GST tag)
A2M 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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别名
- alpha-2-Macroglobulin
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背景
- Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [UniProtKB/Swiss-Prot Function]
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分子量
- 160.8 kDa
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NCBI登录号
- NP_000005
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途径
- Lipid Metabolism
抗原
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