ALAD Protein (Myc-DYKDDDDK Tag)
Quick Overview for ALAD Protein (Myc-DYKDDDDK Tag) (ABIN2714523)
抗原
See all ALAD 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This ALAD protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human ALAD protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Aminolevulinate Dehydratase (ALAD) (AA 1-330) protein (His tag)
custom-made ALAD 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aminolevulinate Dehydratase (ALAD) (AA 1-330) protein (His tag)custom-made ALAD 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Aminolevulinate Dehydratase (ALAD) (AA 1-354) protein (His tag)ALAD 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Aminolevulinate Dehydratase (ALAD) (AA 1-339) protein (GST tag)ALAD 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ALAD (Aminolevulinate Dehydratase (ALAD))
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别名
- Alad
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背景
- The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
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分子量
- 36.1 kDa
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NCBI登录号
- NP_000022
抗原
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