Calreticulin Protein (CALR) (Myc-DYKDDDDK Tag)
Quick Overview for Calreticulin Protein (CALR) (Myc-DYKDDDDK Tag) (ABIN2713072)
抗原
See all Calreticulin (CALR) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Calreticulin protein is labelled with Myc-DYKDDDDK Tag.
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产品特性
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- Recombinant human Calreticulin protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Calreticulin (CALR) protein (His tag)
CALR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % (SDS-PAGE) SDS
Calreticulin (CALR) (AA 25-199) protein (His-IF2DI Tag)CALR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
Calreticulin (CALR) (AA 1-417) protein (GST tag)CALR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
说明
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The tag is located at the C-terminal.
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限制
- 仅限研究用
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浓度
- 50 μg/mL
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缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Calreticulin (CALR)
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别名
- Calreticulin
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背景
- Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
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分子量
- 46.4 kDa
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NCBI登录号
- NP_004334
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途径
- Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
抗原
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