H-2Ld:Ig Fusion Protein 蛋白
This Recombinant H-2Ld:Ig Fusion Protein protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.
(2 references)
产品编号 ABIN2688829
发货至:
中国
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Quick Overview for H-2Ld:Ig Fusion Protein 蛋白 (ABIN2688829)
抗原
H-2Ld:Ig Fusion Protein
蛋白类型
Recombinant
宿主
小鼠
资源
大肠杆菌（E. Coli）
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产品特性
- The H-2Ld:Ig fusion protein consists of three extracellular major histocompatibility complex (MHC) class I H-2Ld domains that are fused to the VH regions of mouse IgG1 (see Figure 1). In order for the MHC class I to be functional, i.e., capable of binding peptides, β2 Microglobulin (β2M) must be present. For this reason, the BD Pharmingen DimerX consists of recombinant H-2Ld:Ig fusion protein, supplemented with recombinant β2M. Recombinant MHC molecules, like the DimerX fusion protein, are useful for studying T-cell function by immunofluorescent staining and flow cytometric analysis of antigen-specific T cells. The MHC gene locus encodes a group of highly polymorphic, cell-surface proteins that play a broad role in the immune response to protein antigens. MHC molecules function by binding and presenting small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Human (human leukocyte antigen/HLA) and mouse (histocompatibility 2/H-2) MHC molecules are structurally and functionally related proteins that comprise two major classes. Class I MHC molecules consist of two separate polypeptide chains. The class I α chain is an MHC encoded, transmembrane polypeptide containing three extracellular domains: α1, α2, and α3. The second chain consists of a non-MHC encoded polypeptide called β2M. Since β2M does not contain a transmembrane domain, it associates with the α chain through noncovalent interaction. Functionally, class I MHC molecules can bind peptides derived from intracellular antigens (e.g., viral and some bacterial antigens) that are specifically recognized by CD8+ T cells. Class II MHC molecules consist of two different transmembrane proteins that can bind peptide fragments derived from extracellular proteins (e.g., bacteria and fungi) and are specifically recognized by CD4+ T cells. TCR recognize both processed peptides bound to MHC, as well as regions of the MHC molecule itself. CD4 and CD8 accessory molecules strengthen formation of the TCR-MHC complex through their interaction with non-polymorphic regions of the MHC molecule.
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纯化方法
- The H-2Ld protein was expressed together with human β2M in the mouse plasmacytoma cell line, J558L (ATCC TIB-6). The H-2Ld and β2M polypeptide chains are associated noncovalently as a consequence of their coexpression within J558L cells. The H-2Ld:Ig fusion protein was purified from tissue culture supernatant by affinity chromatography.
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说明
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BD™ DimerX:Recombinant Soluble Dimeric Mouse H-2Ld:Ig Fusion Protein - Purified
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Probing the activation requirements for naive CD8+ T cells with Drosophila cell transfectants as antigen presenting cells." in: Immunological reviews, Vol. 165, pp. 249-65, (1999) (PubMed).
: "Requirements for peptide-induced T cell receptor downregulation on naive CD8+ T cells." in: The Journal of experimental medicine, Vol. 185, Issue 4, pp. 641-51, (1997) (PubMed).
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- H-2Ld:Ig Fusion Protein
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Sub Type
- Fusionprotein
抗原
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