LIF Protein (AA 23-202)
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- 抗原 See all LIF 蛋白
- LIF (Leukemia Inhibitory Factor (LIF))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 23-202
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Immunofluorescence (IF)
- 纯度
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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- Discover our top product LIF 蛋白
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (HIS-GST)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Leukemia Inhibitory Factor (LIF) (AA 24-203) protein (His tag)LIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His tag)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) proteinLIF 宿主: 人, 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % (SDS-PAGE) SDS Active
Leukemia Inhibitory Factor (LIF) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized, carrier-free.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- LIF (Leukemia Inhibitory Factor (LIF))
- 别名
- LIF (LIF 产品)
- 别名
- CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein
- 背景
- Leukemia inhibitory factor (LIF) is a 20 kD protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF displays diverse biological effects but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. Human and mouse LIF share 79 % sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse specific. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulation of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. LIF can be upregulated by pro-inflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.
- 分子量
- The 180 amino acid recombinant protein has a predicted molecular mass of 19.6 kDa. The predicted N-terminal amino acid is Ser.
- 途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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