IL-22 Protein (AA 34-179, N-Term)
-
- 抗原 See all IL-22 (IL22) 蛋白
- IL-22 (IL22) (Interleukin 22 (IL22))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- N-Term, AA 34-179
-
宿主
- 人
-
资源
- 大肠杆菌(E. Coli)
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- 内毒素水平
-
Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product IL22 蛋白
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Biological activity: ED50 = 0.7 - 1.0 ng/ml, corresponding to a specific activity of 1.0 - 1.4 x 106 units/mg as determined by the dose-dependent stimulation of human Colo205 cells in production of IL-10.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized, carrier-free.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
-
- 抗原
- IL-22 (IL22) (Interleukin 22 (IL22))
- 别名
- IL-22 (IL22 产品)
- 别名
- IL-21 Protein, IL-22 Protein, IL-D110 Protein, IL-TIF Protein, ILTIF Protein, TIFIL-23 Protein, TIFa Protein, zcyto18 Protein, IL-22a Protein, ILTIFa Protein, Iltif Protein, RGD1561292 Protein, interleukin-22 Protein, interleukin 22 Protein, IL22 Protein, il22 Protein, Il22 Protein
- 背景
- IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79 % amino acid identity with mouse IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4+ effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.
- 分子量
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16.9 kDa. The predicted N-terminal amino acid is Met.
-