IL17F Protein (AA 29-161)
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Quick Overview for IL17F Protein (AA 29-161) (ABIN2667481)
抗原
See all IL17F 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 29-161
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 17F (IL17F) (AA 31-163) protein (His-Avi Tag,Biotin)
IL17F 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 29-161) protein (His-Avi Tag)IL17F 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 29-161) protein (His-Avi Tag,Biotin)IL17F 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 31-163) protein (His tag)IL17F 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 31-163) (Active) protein (His tag)IL17F 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 17F (IL17F) (AA 31-163) (Active) protein (His tag)IL17F 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 17F (IL17F) protein (His tag)IL17F 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 17F (IL17F) (AA 28-161) protein (His tag)IL17F 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 17F (IL17F) (Active) protein (His tag)IL17F 宿主: 大鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
Interleukin 17F (IL17F) (AA 1-163) protein (GST tag)IL17F 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 =300 to 600 ng/ml, corresponding to a specific activity of 0.16 - 0.33 x 104 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of CXCL1.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL17F (Interleukin 17F (IL17F))
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别名
- IL-17F
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背景
- IL-17F belongs to the IL-17 cytokine family which includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the strongest homology to IL-17A. They share 50 % amino acid sequence homology. Recently, an IL-17-IL-17F heterodimer was found to be expressed in Th17 cells together with IL-17 and IL-17F homodimers. IL-17 and IL-17F gene promoters share the same pattern of chromatin remodeling in differentiated Th17 cells. T cell populations in vitro and in vivo have shown different ratios of IL-17 and IL-17F expression. IL-17, IL-17F regulates proinflammatory gene expression in vitro. Studies in vivo using Il17a(-/-), Il17f(-/-), and Il17a(-/-)Il17f(-/-) mice showed that IL-17F played only marginal roles, if at all, in the development of delayed-type and contact hypersensitivities, autoimmune encephalomyelitis, collagen-induced arthritis, and arthritis in Il1rn(-/-) mice. In contrast, both IL-17F and IL-17A were involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. Additional studies in vivo have suggested a role for IL-17F in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation.
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分子量
- The 134 amino acid recombinant protein has a predicted molecular mass of 15014 Da and contains Methionine as N-terminal. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15 kDa by SDS-PAGE. The non-red
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
抗原
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