IL17F Protein (AA 31-163)
Quick Overview for IL17F Protein (AA 31-163) (ABIN2667479)
抗原
See all IL17F 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 31-163
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01ng per μg cytokine as determined by the LAL method.
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Interleukin 17F (IL17F) (AA 31-163) protein (His-Avi Tag,Biotin)
IL17F 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 29-161) protein (His-Avi Tag)IL17F 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 31-163) protein (His tag)IL17F 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 29-161) protein (His-Avi Tag,Biotin)IL17F 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 31-163) protein (His tag)IL17F 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17F (IL17F) (AA 31-163) (Active) protein (His tag)IL17F 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 17F (IL17F) (AA 31-163) (Active) protein (His tag)IL17F 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 17F (IL17F) protein (His tag)IL17F 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 17F (IL17F) (AA 28-161) protein (His tag)IL17F 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 17F (IL17F) (Active) protein (His tag)IL17F 宿主: 大鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 =400 - 800 ng/ml, corresponding to a specific activity of 2.5 - 1.25 x 103 units/mg, as determined by the dose dependent stimulation of CXCL1 production in human fibroblast.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL17F (Interleukin 17F (IL17F))
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别名
- IL-17F
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背景
- IL-17F belongs to the IL-17 cytokine family which includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the strongest homology to IL-17A. They share 50 % amino acid sequence homology. The genes encoding IL-17 and IL-17F are localized in the same chromosomal region and are co-expressed by CD4+ and gamma delta T cells. Recently, an IL-17-IL-17F heterodimer was found to be expressed in Th17 cells together with IL-17 and IL-17F homodimers. Similar to IL-17, IL-17F utilizes IL-17RA and IL-17RC as its receptor and employs Act1 and TRAF6 as its signal transducers to induce the expression of pro-inflammatory cytokines and chemokines in many different cell types. IL-17F expression is upregulated in inflammatory bowel disease. A His161 to Arg161 (H161R) substitution in the third exon of the IL17F gene seems to be associated with asthma and chronic obstructive pulmonary disease (COPD) in Japanese subjects. In addition polymorphism of IL-17F seems to be associated to susceptibility to gastric cancer.
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分子量
- The 133 amino acid recombinant protein has a predicted molecular mass of 14903.1 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer,
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
抗原
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