IL-17A/F Protein (Heterodimer)
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北京 101111
Quick Overview for IL-17A/F Protein (Heterodimer) (ABIN2667474)
抗原
See all IL-17A/F 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Heterodimer
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过滤
- 0.22 μm filtered
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内毒素水平
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Endotoxin level is < 0.1 EU/μg (< 0.01 ng/μg) protein as determined by the LAL method.
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Interleukin-17 A/F Heterodimer (IL-17A/F) (AA 26-158) protein (His-Avi Tag)
IL-17A/F 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin-17 A/F Heterodimer (IL-17A/F) (AA 24-155) protein (His tag)IL-17A/F 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin-17 A/F Heterodimer (IL-17A/F) (AA 26-158) protein (His-Avi Tag,Biotin)IL-17A/F 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin-17 A/F Heterodimer (IL-17A/F) (AA 24-155) protein (His-Avi Tag,Biotin)IL-17A/F 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin-17 A/F Heterodimer (IL-17A/F) proteinIL-17A/F 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Interleukin-17 A/F Heterodimer (IL-17A/F) proteinIL-17A/F 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Interleukin-17 A/F Heterodimer (IL-17A/F) proteinIL-17A/F 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Interleukin-17 A/F Heterodimer (IL-17A/F) proteinIL-17A/F 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Interleukin-17 A/F Heterodimer (IL-17A/F) proteinIL-17A/F 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Interleukin-17 A/F Heterodimer (IL-17A/F) proteinIL-17A/F 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Activity was tested by induction of IL-6 in human skin fibroblasts by IL-17A/F. The ED50 is 15-25 ng/ml, corresponding to a specific activity of 6.6 - 4 x 104 units/mg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 300 mM NaCl, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))
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别名
- IL-17A/F
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背景
- IL-17A/F is part of the IL-17 cytokine family which consists of six structurally related proteins (IL-17A, B, C, D, E, and F). IL-17A is expressed primarily by Th17 cells, a subset of CD4 T cells. IL-17F is most closely related to IL-17A. The two molecules share 50 % aminoacid sequence homology. Like IL-17A, IL-17F mRNA and protein have been detected in Th17 cells. IL-17F and IL-17A exist as a homodimers, adopting a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for the interchain bonding. IL-17F and IL-17A can form both homodimeric and heterodimeric proteins when expressed in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. In addition, activated human CD4-T cells produce the homodimers of IL-17F, IL-17A, and the IL-17F/IL-17A heterodimer.
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分子量
- Recombinant hIL-17A/F is a disulfide linked heterodimer consisting of N-terminal methionylated hIL-17A and non-methionylated hIL-17F. The recombinant heterodimer has a predicted molecular mass of 30,552 Da. The DTT-reduced protein migrates as monomers, at
抗原
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