Heregulin beta 1 Protein (AA 177-241)
This Recombinant Heregulin beta 1 protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for Heregulin beta 1 Protein (AA 177-241) (ABIN2667430)
抗原
Heregulin beta 1
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Flow Cytometry (FACS)
纯度
>98 % , as determined by Coomassie stained SDS-PAGE.
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产品特性
- AA 177-241
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内毒素水平
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Less than 0.1 ng per μg of protein.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg, as measured by its ability to stimulate proliferation of human MCF7 cells in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized, carrier-free.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- Heregulin beta 1
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别名
- Heregulin-Beta1
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背景
- Heregulin, also named neuregulin-1 type I, is a family of growth factors that act as ligands for the epidermal growth factor receptors, including ERBB3 and ERBB4. Heregulins regulate survival, proliferation, and differentiation of the cells during development and wound healing. Heregulin-β1 is one of the Heregulin isoforms derived from alternative splicing. Binding of Heregulin-β1 to receptor tyrosine kinases ERBB3 or ERBB4 results in the recruitment and activation of ERBB2, subsequently transducing signaling cascades through the mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3 kinase (PI3K) pathways. It has been suggested that heregulin-β1 participates in the tumorgenesis and metastasis of breast cancer, promoting proliferation, migration, and invasion of breast cancer cells.
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分子量
- The 65 amino acid recombinant protein has a predicted molecular mass of approximately 7.5 kDa. The predicted N-terminal acid is Ser.
抗原
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