G-CSF Protein (AA 27-200)
Quick Overview for G-CSF Protein (AA 27-200) (ABIN2667413)
抗原
See all G-CSF (CSF3) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 27-200
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内毒素水平
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Less than 0.1 ng per μg of protein.
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Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) protein
CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-208) (Active) proteinCSF3 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) protein (His tag)CSF3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 22-214) protein (His-IF2DI Tag)CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) protein (GST tag)CSF3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg as determined by a cell proliferation assay using mouse NFS-60 cells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized, carrier-free.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- G-CSF
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背景
- Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil development. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9. This in turn degrades important HSC trafficking and adhesion molecules, such as c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T cells. The receptor for G-CSF is expressed not only in hematopoietic cells, it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.
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分子量
- The 174 amino acid recombinant protein has a predicted molecular mass of 18.6 kDa. The predicted N-terminal amino acid is Thr.
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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