FGF-1-Acidic (AA 16-155), (N-Term) (Active) 蛋白
This Recombinant FGF-1-Acidic protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN2667397
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for FGF-1-Acidic (AA 16-155), (N-Term) (Active) 蛋白 (ABIN2667397)
抗原
FGF-1-Acidic
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Intracellular Flow Cytometry (ICFC)
纯度
>97 % , as determined by Coomassie stained SDS-PAGE.
-
-
产品特性
- AA 16-155, N-Term
-
过滤
- 0.22 μm filtered
-
内毒素水平
-
Less than 0.01 ng per μg cytokine as determined by the LAL method.
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Biological activity: The ED50 is 0.2 - 1.0 ng/ml, corresponding to a specific activity 1.0 - 5.0 x 106 units/mg, as determined by a dose-dependent stimulation of NIH3T3 cell proliferation in the presence of 10 μg/ml heparin.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.
-
缓冲液
- 0.22 μm filtered protein solution is in 20 mM MOPS, 100 mM NaCl, pH 7.0.
-
注意事项
- Avoid repeated freeze/thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- Unopened vial can be stored at -20°C for three months or at -70°C for 6 months.
-
-
- FGF-1-Acidic
-
背景
- FGF-1, one of the most studied members of the fibroblast growth factor family, is a powerful mitogen exhibiting strong action on many different cell types. FGF-1 activity can be mediated not only by autocrine/paracrine pathways but also by an intracrine pathway. FGF-1 lacks a secretion signal peptide and is exported through a non-classical pathway. Endogenous FGF-1 is found in the nucleus of most cell types. Nuclear localization is required for FGF-1 mitogenic activity. FGF-1 promotes tumor development by promoting cancer cell proliferation and survival. Increased FGF-1 expression in early stages of many different cancers has been reported. MCF-7 breast cancer cell line overexpressing FGF-1 can form vascularized, metastatic tumors when injected into ovariectomized or tamoxifen-treated nude mice. FGF-1 also induces angiogenesis in vitro and in vivo. Thus, FGF-1 is an attractive candidate for cancer immunotargeting. FGF-1 is also involved in neuronal cell differentiation and survival. FGF-1 is highly expressed in motor neurons. In response to damage, motor neurons can release FGF-1 which results in astrocyte activation. Under oxidative stress, astrocytes can also release FGF-1 which stimulates ApoE/HDL generation in an autocrine manner for protection of the brain against oxidative stress. Involvement of FGF-1 in inflammation, cardioprotection, wound healing, adipocyte remodeling, and restenosis is also reported.
-
分子量
- The 140 amino acid recombinant protein has a predicted molecular mass of approximately 16 kDa. The DTT-reduced and non-reduced protein migrates at approximately 17 kDa by SDS-PAGE. The predicted N-terminal amino acid is Met.
抗原
-
You are here:
