Cathepsin E Protein (CTSE) (AA 18-396)
Quick Overview for Cathepsin E Protein (CTSE) (AA 18-396) (ABIN2667383)
抗原
See all Cathepsin E (CTSE) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 18-396
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Cathepsin E (CTSE) (AA 60-397) protein (His tag)
CTSE 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Cathepsin E (CTSE) (AA 18-396) protein (GST tag)CTSE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin E (CTSE) (AA 21-397) protein (His tag)CTSE 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
Cathepsin E (CTSE) (AA 57-363) protein (His tag)CTSE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
Cathepsin E (CTSE) protein (His tag)CTSE 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Cathepsin E (CTSE) (AA 1-397) protein (His tag)CTSE 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: After auto-activation, CTSE activity is determined by its ability to cleave the fluorogenic peptide substrate, Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. The specific activity is >1,500 pmol/min/μg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM MES, 150 mM NaCl, pH 6.5.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -70°C for six months.
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- Cathepsin E (CTSE)
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别名
- Cathepsin E
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背景
- Cathepesin E (CTSE) is an intracellular aspartic protease that was originally identified as a cathepsin D-like acid protease. CTSE and CTSD have similar substrate specificities and CTSE is active in acidic conditions in a pH range from 2.5 to 5.5. In vitro experiments have identified several CTSE substrates including insulin beta chain, neurokinin, and FGF. Although the function of CTSE is not completely understood, it has been implicated in several physiological and pathological processes. CTSE is required for antigen presentation on class II MHC molecules and subsequently, CTSE-deficient mice have increased susceptibility to bacterial infections. CTSE-deficient macrophages also show abnormalities, such as autophagy. Like many other cathepsins, CTSE has emerged as a therapy target for cancers, such as pancreatic ductal adenocarcinoma (PDAC). In addition to PDAC, CTSE is also overexpressed in gastric carcinomas and cervical and lung adenocarcinomas. The possible involvement of CTSE in neurodegeneration has also been reported.
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分子量
- The 389 amino acid recombinant protein has a predicted molecular mass of approximately 42.3 kDa. The DTT-reduced protein migrates at approximately 45 kDa and the non-reduced protein migrates at approximately 90 kDa by SDS-PAGE. The N-terminal amino acid i
抗原
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