Quick Overview for IGF2 Protein (AA 25-91) (ABIN2666937)
抗原
See all IGF2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 25-91
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 1.0 EU per μg cytokine as determined by the LAL method.
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Insulin-Like Growth Factor 2 (IGF2) (AA 1-113) protein (GST tag)
IGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Insulin-Like Growth Factor 2 (IGF2) (AA 1-180) protein (GST tag)IGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Insulin-Like Growth Factor 2 (IGF2) proteinIGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Insulin-Like Growth Factor 2 (IGF2) (AA 25-91) (Active) protein (Fc Tag)IGF2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Insulin-Like Growth Factor 2 (IGF2) proteinIGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Insulin-Like Growth Factor 2 (IGF2) (AA 30-180), (partial) protein (GST tag)IGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
Insulin-Like Growth Factor 2 (IGF2) (AA 25-91) (Active) protein (AVI tag,Fc Tag,Biotin)IGF2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 10 - 30 ng/ml, corresponding to a specific activity of 0.3 - 1 x 105 units/mg, as determined by the dose dependent stimulation of MCF-7 cell proliferation.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in 5 mM citric acid, 5 mM NaHPO4 , 0.15 M NaCl, pH 3.0.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- IGF2 (Insulin-Like Growth Factor 2 (IGF2))
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别名
- IGF-II
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背景
- IGF-II is a member of the IGF family that is comprised of three members (IGF-I, IGF-II, and insulin). IGF-I and IGF-II share approximately 50 % similarity with insulin at the amino acid level. IGF-II is synthesized as a 180 amino acid in the pro-IGF-II form, pro-IGF-II is processed to the mature, active protein with the help of glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-II. GRP94 and IGF-II play a key role in muscle differentiation. Seven binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half life and regulate the availability of the IGFI and IGFII. It has been described that IGFBP3 is the main circulating binding protein for IGFs, in serum approximately 75 % of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-II binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration as well as metabolic function of IGF-II. Embryonic stem cell differentiation into endothelial cells is promoted by the binding of IGF-II to IGFR1. IGF-II binding to IGF-IIR induces internalization and clearing of IGF-II. Osteogenic differentiation and bone formation is enhanced by IGF-II through bone morphogenetic protein-9 (BMP-9). Polymorphisms of IGF-II are associated with obesity, cardiovascular risk factors, and hypertension.
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分子量
- The 67 amino acid recombinant protein has a predicted molecular mass of approximately 7.4 kDa. The DTT-reduced and non-reduced protein migrate at approximately 9 kDa by SDS-PAGE. The N-terminal amino acid is Ala.
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途径
- Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
抗原
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