CXCL12 Protein (AA 22-93)
Quick Overview for CXCL12 Protein (AA 22-93) (ABIN2666911)
抗原
See all CXCL12 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 22-93
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 23-89) (Active) protein (His tag,GST tag)
CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 1-89) protein (GST tag)CXCL12 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 24-89) (Active) protein (Biotin)CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE FACS, CMA Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its ability to chemoattract THP-1 cells in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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别名
- CXCL12
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背景
- CXCL12 is commonly known as SDF-1, with two variants, SDF-1α (89 amino acids) and SDF-1β (93 amino acids) derived from alternative splicing. They have identical amino acid sequences, differing only by the addition of four amino acids at the carboxy-terminus of the β form. CXCL12β is constitutively expressed by stromal cells. CXCL12α and β are ubiquitously expressed, and it has been suggested that their activity is regulated through differential proteolytic processing. Proteases present in serum process CXCL12α initially at the carboxy-terminus (through carboxypeptidase N) and subsubsequently at the amino-terminus (through CD26), while CXCL12β is only processed at the amino-terminus. These proteolytic processes affect the chemokine's ability to bind to heparin and cells as well as its ability to stimulate proliferation and chemotaxis. CXCL12α is inactivated by other enzymes such as metalloproteases, serine proteases, and leukocyte elastase. CXCL12/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Also, CXCL12 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. Data suggests that CXCL12β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells.
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分子量
- The 72 amino acid recombinant protein has a predicted molecular mass of approximately 8.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 11 and 14 kDa by SDS-PAGE respectively. The N-terminal amino acid is Lysine.
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途径
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
抗原
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