Ccl12 Protein (AA 23-104)
Quick Overview for Ccl12 Protein (AA 23-104) (ABIN2666885)
抗原
See all Ccl12 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 23-104
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-C Motif) Ligand 12 (Ccl12) (Active) protein
Ccl12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-C Motif) Ligand 12 (Ccl12) (AA 23-104) protein (His tag)Ccl12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-C Motif) Ligand 12 (Ccl12) proteinCcl12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） >95 %
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its ability to chemoattract THP-1 cells in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
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别名
- CCL12
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背景
- Mouse CCL12 belongs to the CC chemokine family and is most closely related to human CCL2 (MCP-1) in structure (66 % amino acid identity). CCL12 binds to cell surface receptor CCR2, a human CCL2 primary receptor, and can induce migration and Ca2+ flux. CCL12 is a potent chemoattractant for peripheral blood monocytes but only has weak activity on eosinophils at high doses. CCL12 is produced by macrophages during early allergic reactions and infections. During murine MCV infection, bone marrow F4/80 (+) cells can produce high levels of CCL12. The expression of CCL12 can also be upregulated by bacterial infections including pneumococcal meningitis and polymicrobial septic peritonitis. LPS can induce the expression of CCL12 in liver macrophages (Kupffer cells) in vivo. CCL12 is involved in the pathogenesis of pulmonary fibrosis by recruiting fibrocytes to the injury site and enhancing fibrotic responses. Also, CCL12 is implicated in liver and kidney fibrosis. It has also been shown that hypoxia inducible factor 1 is involved in hypoxia-induced upregulation of CCL12 in astrocytes. In addition, CCL12 is involved in joint and growth plate development.
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分子量
- The 82 amino acid recombinant protein has a predicted molecular mass of approximately 10 kDa. The DTT-reduced protein migrates at approximately 13 kDa and non-reduced protein migrates at approximately 15 kDa by SDS-PAGE. The N-terminal amino acid is Gly.
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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