IFNA2 Protein (AA 24-188)
Quick Overview for IFNA2 Protein (AA 24-188) (ABIN2666827)
抗原
See all IFNA2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 24-188
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interferon, alpha 2 (IFNA2) (Active) protein
IFNA2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon, alpha 2 (IFNA2) proteinIFNA2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interferon, alpha 2 (IFNA2) (AA 1-188) protein (GST tag)IFNA2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interferon, alpha 2 (IFNA2) (AA 24-188) protein (Fc Tag)IFNA2 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Specific activity is 1.86 x 108 units/mg, as determined in a cytopathic effect inhibition assay using EMC virus on A549 cells.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- IFNA2 (Interferon, alpha 2 (IFNA2))
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别名
- IFN-Alpha2
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背景
- Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. Also, they are highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). Also, IFN-α2 induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α had been used in the treatment of chronic hepatitis C (CHC), nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α, therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.
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分子量
- The 166 amino acid recombinant protein has a predicted molecular mass of approximately 19.4 kDa. The DTT-reduced and non-reduced proteins migrate at approximately 20 kDa and 18 kDa respectively by SDS-PAGE. The N-terminal amino acid is Met.
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C
抗原
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