GM-CSF Protein (AA 18-144)
Quick Overview for GM-CSF Protein (AA 18-144) (ABIN2666817)
抗原
See all GM-CSF (CSF2) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 18-144
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein
CSF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)CSF2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 宿主: 人 宿主: HEK-293 Cells > 95 % AcA, CC Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 5 - 20 pg/ml, corresponding to a specific activity of 5 - 20 x 107 units/mg, as determined by induction of FDC-P1 cell proliferation.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- GM-CSF
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背景
- Csf2 is the gene that encodes GM-CSF (granulocyte-macrophage colony stimulating factor), which is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, and rat. GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRalpha) and a major signaling subunit (beta-c). The beta-c chain is a common subunit for GM-CSF, IL-3, and IL-5 receptors. The receptor subunits are always coexpressed on the surface of leukocytes, with beta-c being expressed at lower levels than GMRalpha. Certain nonhemopoietic cell types also express the GM-CSF receptor. GM-CSF has anti-apoptotic functions on neurons and is neuroprotective in animal stroke models. It stimulates neuronal differentiation of adult neuronal stem cells (NSCs). GM-CSF has been identified as a factor involved in arteriogenesis after brain ischemia. In addition, GM-CSF induces arteriogenic growth of collateral vessels after occlusion of cardiac or peripheral arteries.
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分子量
- The 128 amino acid recombinant protein has a predicted molecular mass of approximately 14.6 kDa. The DTT-reduced and non-reduced proteins migrate at approximately 17 and 14 kDa respectively by SDS-PAGE. The N-terminal amino acid is Met.
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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