Quick Overview for IL-9 Protein (AA 19-144) (ABIN2666758)
抗原
See all IL-9 (IL9) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 19-144
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.1 EU per μg of protein as determine by the LAL method.
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Interleukin 9 (IL9) (AA 19-144) protein (Fc Tag)
IL9 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (His tag)IL9 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (Fc Tag)IL9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (His tag)IL9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (Fc Tag)IL9 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 9 (IL9) (AA 19-144) protein (His tag)IL9 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 9 (IL9) (AA 1-144) protein (GST tag)IL9 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Human IL-9 induces proliferation of MO7e cells. ED50=0.1-0.5 ng/mL, corresponding to a specific activity of 2-10 x 106 units/mg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- IL-9 (IL9) (Interleukin 9 (IL9))
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别名
- IL-9
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背景
- IL-9 was initially identified in mice as a T cell growth factor. Human and murine IL-9 show a 56 % amino acid sequence homology. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα ) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15, and IL-21. IL-9 is expressed by activated Th9, Th17, Tregs, and mast cells and appears to play an important role in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immunosuppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a key factor in the pathogenesis of bronchial hyperresponsiveness. In this sense, it has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability and plays a role in the development of food allergies. IL-9 has also been associated with atopic dermatitis.
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分子量
- The 126 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa. The protein migrates as multiple bands with the molecular mass of 18 - 25 kDa band in SDS-PAGE in DTT-reducing conditions due to different glycosylation. The N-
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途径
- JAK/STAT Signaling
抗原
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