IL1F9 Protein (AA 13-164)
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北京 101111
Quick Overview for IL1F9 Protein (AA 13-164) (ABIN2666737)
抗原
See all IL1F9 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 13-164
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 1 Family Member 9 (IL1F9) (AA 13-164) protein (His tag)
IL1F9 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 1 Family Member 9 (IL1F9) (AA 18-169) protein (His tag)IL1F9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 1 Family Member 9 (IL1F9) (AA 1-169) protein (His tag)IL1F9 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 1 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1.0 x 106 units/mg, as determined by a dose-dependent stimulation of IL-6 secretion in 3T3L1 preadipocytes.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM MOPS, pH 7.4, 150 mM NaCl, 10 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one week, at -20°C for three months, or at -70°C for 6 months.
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- IL1F9 (Interleukin 1 Family Member 9 (IL1F9))
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别名
- IL-36 gamma
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背景
- The IL-1 family is a group of cytokines comprised of 11 members, including the recently renamed IL-36 cytokines, IL-36α, β, and γ (previously known as IL-1F6, IL-1F8, and IL-1F9, respectively). IL-36γ signals through the IL-1 receptor family members IL-1Rrp2 and IL-1RAcP and can activate downstream MAP kinases and transcription factor NF-κB. IL-36γ is synthesized without signal peptide and the secretion pathway is unclear. It has been shown that like other IL-36 cytokines, the N-terminal processing of IL-36γ can significantly increase its biological activity. The possible processing enzymes are currently unknown. IL-36γ plays a significant role in the immune response, as low levels of IL-36 receptor IL-1Rrp can be detected on bone marrow-derived dendritic cells and CD4+ T cells. IL-36γ treatment can upregulate CD80, CD86 and MHC Class II, and proinflammatory cytokine production in dendritic cells. IL-36γ has also been implicated in airway hyperesponsiveness. Intranasal administration of recombinant IL-36γ in mice leads to epithelial cell hypertrophy, cellular infiltration, and mucus production. IL-36γ expression in the lungs of mice can be upregulated by allergic inflammation. In the skin, IL-36γ is primarily expressed by keratinocytes and its expression can be upregulated by inflammatory cytokines, toll-like receptor ligands, and some pathological conditions. IL-36γ is highly induced in psoriasis plaques.
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分子量
- The 152 amino acid recombinant protein has a predicted molecular mass of approximately 20 kDa. The DTT-reduced and non-reduced protein migrates at approximately 20 kDa by SDS-PAGE. The predicted N-terminal amino acid is Gly.
抗原
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