Lipocalin 2 Protein (LCN2) (AA 21-198, C-Term, His10)
Quick Overview for Lipocalin 2 Protein (LCN2) (AA 21-198, C-Term, His10) (ABIN2666721)
抗原
See all Lipocalin 2 (LCN2) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 21-198, His10, C-Term
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determine by the LAL method.
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Lipocalin 2 (LCN2) (AA 21-198) protein (Fc Tag)
LCN2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lipocalin 2 (LCN2) (AA 21-198) protein (His tag)LCN2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lipocalin 2 (LCN2) (AA 21-198) protein (His tag)LCN2 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lipocalin 2 (LCN2) (AA 21-200) protein (Fc Tag)LCN2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lipocalin 2 (LCN2) protein (His tag)LCN2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Lipocalin 2 (LCN2) (AA 1-198) protein (GST tag)LCN2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Lipocalin 2 (LCN2) (Active) protein (His tag)LCN2 宿主: 大鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
Lipocalin 2 (LCN2) (Active) protein (His tag)LCN2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: BC50 ≥ 1.2 μM, by dose dependent binding of the protein with [ Fe3+(DHBA)3] complex.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for three months, or at -70°C for six months.
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- Lipocalin 2 (LCN2)
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别名
- NGAL
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背景
- NGAL, also known as lipocalin 2, LCN2, and siderocalin, is a small molecule composed of 178 amino acids. It belongs to the lipocalin superfamily of 20 structurally related secreted proteins. Members of the lipocalin family share a highly conserved fold with an eight stranded antiparallel β barrel, and act as a transporter, carrying small molecules to specific cells. NGAL occurs predominantly in a monomeric form, with a small percentage occurring as a dimer or trimer. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. It is a critical component of innate immunity because its ability to bind iron-siderophore complexes limits bacterial growth. Human NGAL is expressed at very low levels in human tissues, including kidney, trachea, lungs, stomach, and colon, and its expression increases greatly in the presence of inflammation and injured epithelia. In addition to its role in innate immunity, it is a promising biomarker for acute kidney injury that is beginning to be used in clinical practice in addition to research studies.
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分子量
- The 193 amino acid recombinant protein has a predicted molecular mass of approximately 22 kDa. The DTT-reduced and non-reduced protein migrate at approximately 24-28 and 23-26 kDa by SDS-PAGE respectively. The N-terminal amino acid is blocked.
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途径
- Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
抗原
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