TIMP2 Protein (AA 27-220, C-Term)
Quick Overview for TIMP2 Protein (AA 27-220, C-Term) (ABIN2666720)
抗原
See all TIMP2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 27-220, C-Term
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 1.0 EU per μg of protein as determine by the LAL method.
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Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (His tag)
TIMP2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Metalloproteinase Inhibitor 2 (TIMP2) protein (Fc Tag)TIMP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Metalloproteinase Inhibitor 2 (TIMP2) protein (His tag)TIMP2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (GST tag)TIMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Metalloproteinase Inhibitor 2 (TIMP2) (AA 30-220), (partial) protein (GST tag)TIMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Human TIMP-2 inhibits the activity of activated mouse MMP-9 (100 ng/mL). The is IC50 ≤ 34 ng/mL.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, pH 8.0.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for three months, or at -70°C for six months.
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- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
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别名
- TIMP-2
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背景
- TIMPs (Tissue inhibitors of metalloproteinases) are endogenous inhibitors for MMPs (matrix metalloproteinases). The human TIMPs consist of structurally and functionally distinct N- and C-terminal domains, each of which is stabilized by three disulfide bonds. The N-terminal domain binds to the active site of MMPs, inhibiting their proteolytic activities. The bidentate coordination of the zinc ion in the active site of an MMP by the N-terminal α-amino group and carbonyl group of the Cys residue in the "cysteine switch" motif is a key mechanism of inhibition with MMPs. The C-terminal domain is known to bind hemopexin-like domain of pro-MMPs. TIMPs are broad-spectrum inhibitors of MMPs, but there are some differences in specificity among them. Human metalloproteinase inhibitor 2 (TIMP-2) expression in melanocytes is regulated by MITF. Besides inhibitory functions, TIMP-2 also has erythroid potentiating and cell growth promoting activities. Interestingly, TIMP-2-deficient mice demonstrated increased tumor growth, enhanced expression of angiogenic marker αvβ3 in tumor and endothelial cells, and significantly higher serum vascular endothelial growth factor A levels. Tumor-bearing TIMP-2 -/- mice showed a significant number of inflammatory cells in their tumors, upregulation of inflammation mediators, nuclear factor-κB, and Annexin A1 as well as higher levels of serum IL-6. TIMP-2 is widely expressed in many mammalian tissues, notably in the reproductive organs. TIMP-2 is able to promote cell proliferation in a wide range of cell types and may also have an anti-apoptotic function. It has anti-angiogenic activity by preventing endothelial cell migration.
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分子量
- Predicted molecular mass of approximately 22 kDa. The protein migrates at about 25 kDa in DTT-reducing conditions and about 22 kDa in non-reducing condition by SDS-PAGE. The N-terminal amino acid is Cys.
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途径
- cAMP Metabolic Process
抗原
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