IL-2 Protein (AA 21-153)
Quick Overview for IL-2 Protein (AA 21-153) (ABIN2666705)
抗原
See all IL-2 (IL2) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 21-153
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过滤
- 0.22 μm filtered
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内毒素水平
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Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.
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Interleukin 2 (IL2) (AA 21-153) protein
IL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) protein (His tag)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag)IL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag,Biotin)IL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 2 (IL2) (AA 21-153) protein (mFc Tag)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 2 (IL2) (Active) proteinIL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 2 (IL2) (Active) proteinIL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Interleukin 2 (IL2) (AA 21-153) (Active) proteinIL2 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 2 (IL2) (AA 21-153) protein (His tag)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 2 (IL2) (AA 21-153) (Active) protein (Fc Tag,AVI tag,Biotin)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 0.102 - 0.295 ng/ml, corresponding to a specific activity of 9.8 - 3.39 x 106 units/mg, as determined by the dose dependent stimulation of CTLL-2 cells.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 5 mM NaH2PO4, 5 mM citric acid, 150 mM NaCl, pH 4.0.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-2 (IL2) (Interleukin 2 (IL2))
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别名
- IL-2
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背景
- Interleukin 2 was discovered through its function as a T cell growth factor (TCGF). IL-2 binds to IL-2 receptor which is expressed in T and B cells, thymocytes, and NK cells. The IL-2 receptor comprises three distinct components: the α -chain, which is cytokine specific, and the β- and γc-subunits which are share with the IL-15 receptor. In addition, the γ c-subunit is a component of a series of other cytokine receptors, these being members of the γc cytokine receptor family (IL-4, IL-7, IL-9, and IL-21) (3). IL-2 signaling may play a major role in the differentiation of regulatory T cells (4). IL-2, IL-15, and IL-7 can all support NK cell differentiation, nevertheless, analyses of IL-2(-/-), IL-2Rα(-/-) mice fail to exhibit significant defects in NK cell development. This data suggest that IL-2 might have a redundant role in NK cell differentiation (4).
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分子量
- The 134 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 15,396 Da. The DTT-reduced protein migrates at approximately 14kDa and the non-reduced protein migrates with slightly greater mobility by SDS-PAGE.
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原
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