IL-13 Protein (AA 21-132)
Our Local Distributor
北京 101111
Quick Overview for IL-13 Protein (AA 21-132) (ABIN2666703)
抗原
See all IL-13 (IL13) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 21-132
-
过滤
- 0.22 μm filtered
-
内毒素水平
-
Endotoxin level is < 0.1 EU/μg (< 0.01ng/μg) protein as determined by the LAL method.
-
-
Interleukin 13 (IL13) (AA 21-132) protein (His-Avi Tag)
IL13 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE
Interleukin 13 (IL13) (AA 19-132) (Active) protein (His tag)IL13 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 13 (IL13) (Active) proteinIL13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 13 (IL13) (AA 26-131) (Active) protein (His tag)IL13 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 13 (IL13) (AA 21-132) (Active) protein (Biotin,His-Avi Tag)IL13 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 13 (IL13) (AA 19-132) (Active) proteinIL13 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 13 (IL13) (AA 21-132) (Active) protein (Fc Tag)IL13 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 13 (IL13) (AA 21-132) (Active) protein (His tag,AVI tag,Biotin)IL13 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 13 (IL13) protein (mFc Tag)IL13 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 13 (IL13) (Active) proteinIL13 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Biological activity: The ED50 is 1.5 to 3ng/ml, corresponding to a specific activity of 6.6 -- 3.3 x 105units/mg, as determined by the dose dependent stimulation of TF-1 cells proliferation.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
-
缓冲液
- 0.22μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
-
注意事项
- Avoid repeated freeze/thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
-
-
- IL-13 (IL13) (Interleukin 13 (IL13))
-
别名
- IL-13
-
背景
- Human IL-13 was initially cloned from cDNA libraries of activated T cells. IL-13 is an immunoregulatory cytokine secreted predominantly by activated T(H)2 cells, and it is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit, the alpha subunit of the IL-4 receptor (IL-4Ralpha). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. Ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. Interleukin-13 (IL-13), like IL-4, is a cytokine produced by T(H)2 type helper T cells in response to signaling through the T cell antigen receptor and by mast cells and basophils upon cross-linkage of the high-affinity receptor for immunoglobulin E (IgE). IL-13 has been implicated in airway hypersensitivity and mucus hypersecretion, inflammatory bowel disease, and parasitic nematode expulsion.
-
分子量
- The 112 amino acid recombinant protein has a predicted molecular mass of 12,344 Da. The DTT-reduced and non-reduced protein migrate at approximately 9kDa by SDS-PAGE. The N-terminal amino acid is Glycine.
-
途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
抗原
-
