IFN beta 1 (AA 22-182) (Active) 蛋白
This Recombinant IFN beta 1 protein is expressed in HEK-293 Cells.
产品编号 ABIN2666650
发货至:
中国
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中国
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Quick Overview for IFN beta 1 (AA 22-182) (Active) 蛋白 (ABIN2666650)
抗原
IFN beta 1
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
HEK-293 Cells
应用范围
Flow Cytometry (FACS)
纯度
> 98 % , as determined by Coomassie stained SDS-PAGE.
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产品特性
- AA 22-182
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: IFN-β1 bioactivity was measured by its property to protect L929 cells from the cytopathic effect (CPE) of encephalomyocarditis virus (EMCV) in a dose dependent manner. The specific activity is 5.1 x 107 units/mg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IFN beta 1
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别名
- IFN-Beta1
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背景
- IFN-β1 is part of the type I IFN multigene family which includes at least eight subclases: IFN-α, IFN-β, IFN-ε, IFN-κ, IFN-ω, IFN-τ, IFN-δ, and IFN-ζ (limitin). Type I interferons (IFNs) (alpha/beta interferon [IFN-α/β]) are expressed as a first line of defense against viruses and play a critical role in the antiviral response. The antiviral activity of type I IFNs is exerted by different mechanisms, e.g. blockage of viral entry into the cell, control of viral transcription, cleavage of RNA, and preventing translation, therefore, type I IFNs block virus replication. In addition, type I INFs modulate the innate and adaptive immune responses. IFN-α/β induces natural killer cell cytotoxicity and expression of major histocompatibility complex class I on most cells and costimulatory molecules on antigen-presenting cells. Also, type I interferons act directly on CD8 T cells to allow clonal expansion and memory formation in response to viral infection. Plasmacytoid dendritic cells (pDCs) produce high amounts of type I interferons (IFNs) and a variety of inflammatory cytokines and chemokines in response to viral infections. Mice deficient for IFN-β type I IFN receptor (IFNAR) have demonstrated an increased susceptibility to experimental autoimmune encephalomyelitis (EAE). Animal studies have shown that neutralization of IL-23 or the lack of IL-23 p19 gene expression completely ameliorated EAE. These data correlate with the fact that IFN-β has been used in the treatment of multiple sclerosis (MS) in humans, and it has been suggested that IFN-β inhibits human Th17 cell differentiation. It is known that Th17 cells play a central role in the immunopathogenesis of MS, and IL-23 plays a role in the expansion of differentiated TL17 cells in mice and differentiation of Th17 cells in humans.
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分子量
- The 175 amino acid recombinant protein has a predicted molecular mass of approximately 21.2 kDa. The DTT-reduced and non-reduced protein migrates at approximately 36 kDa by SDS-PAGE. The N-terminal amino acid is Ile.
抗原
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