G-CSF Protein (AA 31-208)
-
- 抗原 See all G-CSF (CSF3) 蛋白
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 31-208
-
宿主
- 小鼠
-
资源
- 大肠杆菌(E. Coli)
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
-
Less than 0.01 ng per μg cytokine as determined by the LAL method.
- Top Product
- Discover our top product CSF3 蛋白
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Biological activity: ED50 =0.5 - 3 ng/ml, corresponding to a specific activity of 0.33 - 2.0 x 106 units/mg, as determined by M-NFS-60 cell proliferation induced by mouse G-CSF in a dose dependent manner.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
-
- 抗原
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- 别名
- G-CSF (CSF3 产品)
- 别名
- CSF3 Protein, G-CSF Protein, gcsf Protein, Csfg Protein, MGI-IG Protein, C17orf33 Protein, CSF3OS Protein, GCSF Protein, Gcsf Protein, colony stimulating factor 3 Protein, colony stimulating factor 3 (granulocyte) a Protein, colony stimulating factor 3 (granulocyte) Protein, CSF3 Protein, csf3a Protein, Csf3 Protein
- 背景
- G-CSF is the key hematopoietic cytokine involved in the control of neutrophil production, therefore it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC-trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/ macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells, in addition, it is expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.
- 分子量
- The 179 amino acid recombinant protein has a predicted molecular mass of approximately 19 kDa. The DTT-reduced and non-reduced protein migrate at approximately at 20 kDa by SDS-PAGE. The N-terminal amino acid is Met.
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
-