CXCL5 Protein (AA 45-118)
Quick Overview for CXCL5 Protein (AA 45-118) (ABIN2666646)
抗原
See all CXCL5 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 45-118
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-X-C Motif) Ligand 5 (CXCL5) (Active) protein
CXCL5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) proteinCXCL5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) (AA 49-118) protein (His tag)CXCL5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) (AA 41-114) protein (His tag)CXCL5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) protein (Fc Tag)CXCL5 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) (AA 1-114) protein (GST tag)CXCL5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) (AA 37-110), (partial) protein (His tag)CXCL5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Chemokine (C-X-C Motif) Ligand 5 (CXCL5) (AA 46-117), (partial) protein (His tag)CXCL5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))
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别名
- CXCL5
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背景
- CXCL5 is an ELR+CXC chemokine characterized by a Glu-Leu-Arg (ELR) motif preceding the characteristic CXC sequence. CXCL5 chemoattracts and activates neutrophils during inflammation through the binding to CXCR2. CXCL5 occurs in different NH2- and COOH-truncated forms, and both NH2-terminal and COOH-terminal truncation result in an increase in neutrophil chemotactic potency. CXCL5(8-78) and CXCL5(9-78) induce a higher neutrophil influx in vivo compared with CXCL5(1-78). CXCL5 regulates the availability of binding sites for other ELR+ CXC chemokines released during inflammation, through its interaction with erythrocyte duffy antigen receptor (DARC). As a result, CXCL5 induces the increase of CXCL1 and CXCL2 in a model of pulmonary inflammation. Therefore, CXCL5 acts as a regulator of chemokine scavenging and pulmonary host defense to bacterial infection. Lung epithelial cells express CXCR2, consequently, CXCL5 may modulate neutrophil transepithelial and transendothelial migration. Mouse CXCL5 is the ortholog of human CXCL6.
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分子量
- The 74 amino acid recombinant protein has a predicted molecular mass of approximately 7.98 kDa. The DTT-reduced protein and non-reduced protein migrates at approximately 10 kDa by SDS-PAGE. The N-terminal amino acid is Val.
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity
抗原
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