CXCL2 Protein (AA 28-100)
Quick Overview for CXCL2 Protein (AA 28-100) (ABIN2666642)
抗原
See all CXCL2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 28-100
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Active) protein
CXCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Active) proteinCXCL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Active) proteinCXCL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 1-107) protein (GST tag)CXCL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 35-107) protein (His tag)CXCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 32-100) protein (His tag)CXCL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 28-100) protein (His tag)CXCL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) proteinCXCL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 35-107) protein (His-DYKDDDDK-Strep II Tag)CXCL2 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (AA 28-100) protein (His tag)CXCL2 宿主: 小鼠 宿主: Yeast (Pichia pastoris) Recombinant > 95 % SDS, WB, ELISA, IP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))
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别名
- CXCL2
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背景
- CXCL2 is an ELR CXC chemokine, and it is structurally and functionally related to GRO1 (CXCL1), GRO3 (CXCL3), and interleukin-8 (CXCL8). CXCL2 binds to the CXCR2 receptor, and this receptor is shared with other ELR CXC chemokines (CXCL1, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8). CXCL2 is highly expressed in esophageal squamous cell carcinoma (ESCC), and it has been detected in serum of patients with this disease. Also, CXCL1, CXCL2, and CXCL3 have been shown to be highly expressed in patients with malignant melanoma. In addition, CXCL2 and CXCL1 are up-regulated in the central nervous system in multiple sclerosis (MS) and in the animal model of experimental autoimmune encephalomyelitis (EAE). In EAE, it has been shown that transfer of encephalitogenic CD4+ Th17 cells induce CXCL1 and CXCL2 transcription in the spinal cords of naive, syngeneic recipients. IL-17 is a potent inducer of ELR+ CXC chemokines in MS and EAE. In fact, it has been showed that CXCL1 and CXCL2 are up-regulated in the preclinical and clinical stages of EAE. In addition, it has been shown that CXCL1 and CXCL2 are secreted by ephithelial cells in bone marrow, and CXCL2 is secreted by endothelial cells during G-CSF-induced neutrophil mobilization.
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分子量
- The 73 amino acid recombinant protein has a predicted molecular mass of approximately 7.8 kDa. The DTT-reduced protein migrates at approximately 10 kDa and non-reduced protein migrates at 12 kDa by SDS-PAGE. The N-terminal amino acid is Alanine.
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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