CX3CL1 Protein (AA 25-100)
Quick Overview for CX3CL1 Protein (AA 25-100) (ABIN2666622)
抗原
See all CX3CL1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 25-100
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-336) protein (His tag)
CX3CL1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-341) protein (His tag)CX3CL1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-341) protein (His tag)CX3CL1 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-336) protein (His-Avi Tag,Biotin)CX3CL1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (Active) proteinCX3CL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-100) protein (His tag)CX3CL1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 76-182) protein (His tag)CX3CL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 1-397) protein (GST tag)CX3CL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 1-236) protein (GST tag)CX3CL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 351-449) protein (GST tag)CX3CL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract BaF3-mCX3CR1 transfectants in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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别名
- CX3CL1
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背景
- CX3CL1 is a unique chemokine subclass, synthesized as a transmembrane molecule It consists of an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. CX3CL1 exists in two forms: as a membrane-anchored or as a shed 80-95K glycoprotein. Soluble CX3CL1 is generated by limited proteolysis on the cell surface, and a disintegrin and metallopeptidase 10 (ADAM10) and ADAM17/tumor necrosis factor-α-converting enzyme (ADAM17/TACE) participate in this shedding. It has been suggested that ADAM10 acts in the constitutive shedding, and ADAM17 acts in response to cell activation. CX3CL1 is associated with the development of different diseases such as rheumatoid arthritis (RA), rheumatoid vasculitis (RV), Sjögren's syndrome (SS), systemic lupus erythematosus (SLE), scleroderma, multiple sclerosis, and atherosclerosis. Soluble fractalkine has been detected in the cerebrospinal fluid of patients with neuropsychiatric lupus, and is present at higher levels in the serum of patients with SS, RA, and type 2 diabetes than in control subjects.
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分子量
- The 77 amino acid recombinant protein has a predicted molecular mass of approximately 8.77 kDa. The DTT-reduced protein migrates at approximately 12 kDa and non-reduced protein migrates at 15.5 kDa by SDS-PAGE. The N-terminal amino acid is Met.
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途径
- Synaptic Membrane
抗原
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