CXCL12 Protein (AA 22-89)
Quick Overview for CXCL12 Protein (AA 22-89) (ABIN2666583)
抗原
See all CXCL12 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 22-89
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 23-89) (Active) protein (His tag,GST tag)
CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 1-89) protein (GST tag)CXCL12 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 24-89) (Active) protein (Biotin)CXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE FACS, CMA Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract activated T cells in a dose dependent manner. The ED50 is 10 - 50 ng/ml, corresponding to a specific activity of 0.2 - 1.0 x 105 units/mg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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别名
- CXCL12
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背景
- Mouse cDNA CXCL12 was initially cloned from a bone marrow stromal cell library. CXCL12 and its receptor CXCR4 are involved in the regulation of migration, survival, and development of multiple cell types, including human hematopoietic CD34+/CD38-/low and stromal STRO-1+ stem cells. Stress-induced modulations in CXCL12 and CXCR4 levels participate in recruitment of immature and maturing leukocytes from the BM reservoir to damaged organs as part of the host defense and repair mechanism. In addition, CXCL12 and CXCR4 are key regulators of the stem cell mobilization process to ischemic tissues and therefore crucial for tissue repair. The CXCL12/CXCR4 system is involved in the establishment of organ metastasis in different cancers. Cancer stem cells (CSCs), a small subset of cancer cells, constitute a reservoir of self-sustaining cells with the ability to maintain tumor growth. Most of them express the CXCR4 receptor and respond to a chemotactic gradient of CXCL12, suggesting that CSCs probably represent a subpopulation capable of initiating metastasis. Additional data has shown that CXCL12 binds to a second receptor, CXCR7. This receptor is expressed in endothelial cells and is associated with tumors. CXCL12/CXCR4/CXCR7 axis activation seems to regulate the metastatic process.
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分子量
- The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.9 kDa. The DTT-reduced protein migrates at approximately 10 kDa and the non-reduced protein migrates at approximately 12 kDa by SDS-PAGE. The N-terminal amino acid is
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途径
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
抗原
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