APCS Protein (AA 20-223, C-Term)
Quick Overview for APCS Protein (AA 20-223, C-Term) (ABIN2666528)
抗原
See all APCS 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 20-223, C-Term
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 1.0 EU per μg of protein as determined by the LAL method.
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Amyloid P Component, Serum (APCS) (AA 21-224) protein (Fc Tag)
APCS 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Amyloid P Component, Serum (APCS) (AA 20-223) protein (Fc Tag)APCS 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Amyloid P Component, Serum (APCS) protein (His tag)APCS 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Amyloid P Component, Serum (APCS) (AA 1-223) protein (GST tag)APCS 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Amyloid P Component, Serum (APCS) (AA 17-524) protein (His tag)APCS 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Amyloid P Component, Serum (APCS) protein (His tag)APCS 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Amyloid P Component, Serum (APCS) (Active) protein (His tag)APCS 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Human APCS is able to bind immobilized HDL (50 μg/ml) in a dose dependent manner with a half maximum binding of ED50 = 0.24 - 0.96 μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening.
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缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2° and 8°C for one month, at -20°C for three months, or at -70°C for six months.
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- APCS (Amyloid P Component, Serum (APCS))
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别名
- APCS
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背景
- APCS (amyloid P component, serum), also known as pentraxin 2 (PTX2), is a member of the pentraxin family, which has a characteristic non-covalently linked pentameric organization. This protein is characterized by its calcium-dependent ligand binding and distinctive flattened β-jellyroll structure similar to legume lectins. Human APCS shares a 79 % amino acid homology with its mouse counterpart. APCS is secreted by hepatocytes as part of the acute phase protein synthesys in response to infections. Upon binding to its ligands, APCS is involved in the activation of classical complement cascade. In a mouse model, APCS was shown to downregulate acute inflammation accompanying experimental autoimmune encephalomyelitis by regulating T cell activity. Besides its role in regulating immune response, APCS is found to be a component of amyloid deposits, which has the ability of stabilizing amyloid fibrils and protecting them from proteolytic degradation. Based on these studies, it was postulated that APCS may play a role in Alzheimer's disease and other amyloidoses. Interestingly, APCS is also identified as a component of basement membrane proteins, and is able to bind laminin, collagen IV, P-selectin, and fibronectin in a Ca2+ dependent manner. In addition, APCS is known to specifically localize in human atherosclerotic lesions, which suggests that it may play a role in atherogenesis. Functional studies revealed that APCS binds to lipoproteins, specifically to HDL, but not LDL.
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分子量
- The 219 amino acid recombinant protein has a predicted molecular mass of approximately 25 kDa. The protein migrates at 27 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.
抗原
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