MMP8 Protein (AA 21-467, C-Term)
Quick Overview for MMP8 Protein (AA 21-467, C-Term) (ABIN2666511)
抗原
See all MMP8 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 21-467, C-Term
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 1.0 EU per μg of protein as determined by the LAL method.
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Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) protein (His tag)
MMP8 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) protein (His tag)MMP8 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-465) protein (His tag)MMP8 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 1-467) protein (GST tag)MMP8 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) proteinMMP8 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Activated human MMP-8 is able to cleave the peptide substrate Mca-KPLGL-Dpa-AR-NH2 with an activity above 375 pmol/min/μg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening.
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缓冲液
- 0.22 μm filtered protein solution is in TCN (25 mM TRIS, 10 mM CaCl2, 150 mM NaCl, pH 7.5).
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -70°C for six months.
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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别名
- MMP-8
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背景
- MMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. MMP-8 consists of a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a hinge region, and a carboxyl terminal domain with hemopexin-like repeats. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.
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分子量
- This 460 amino acid recombinant protein has a predicted molecular mass of approximately 52.6 kDa. The protein migrates at approximately 70 kDa in DTT-reducing conditions and approximately 70 kDa in non-reducing conditions by SDS-PAGE.The predicted N-termi
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