MMP2 Protein (AA 34-662, C-Term)
Quick Overview for MMP2 Protein (AA 34-662, C-Term) (ABIN2666505)
抗原
See all MMP2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- C-Term, AA 34-662
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.1 EU per μg of protein as determined by the LAL method.
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Matrix Metalloproteinase 2 (MMP2) (Active) protein
MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Matrix Metalloproteinase 2 (MMP2) (AA 424-660) protein (His-IF2DI Tag)MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 1-660) protein (GST tag)MMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Matrix Metalloproteinase 2 (MMP2) (AA 109-660) protein (His tag)MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 30-660) protein (His tag)MMP2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Mouse MMP-2 cleaves the peptide substrate Mca-PLGL-Dpa-AR-NH2 with an activity above 1400 pmol/min/μg.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening.
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缓冲液
- 0.22 μm filtered protein solution is in TCN (25 mM TRIS, 5 mM CaCl2, 150 mM NaCl, pH 7.5).
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -70°C for six months.
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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别名
- MMP-2
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背景
- MMP-2, also known as gelatinase A, is a member of matrix metalloproteinase family proteins (MMPs). MMPs are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling as well as in disease processes such as arthritis and metastasis. MMP-2 consists of a prodomain, which is cleaved upon activation, a catalytic domain containing the zinc binding site, a fibronectin-like domain (that plays a role in the substrate targeting), and a carboxyl terminal (hemopexin-like repeats) domain. Activation of MMP-2 requires proteolytic processing: a complex of membrane type 1 MMP (MT1-MMP) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP-2 from the extracellular milieu to the cell surface. Next, the MMP-2 is activated by active MT1-MMP and subsequently undergoes auto-catalytic cleavage. Substrates of MMP-2 include type IV collagen, aggrecan, link protein, decorin, fibronectin, and type X and XI collagens, all of which are components of the articular cartilaginous matrix. Importantly, MMP-2 secretion is elevated in several types of human cancers and its elevated expression has been associated with poor prognosis. Mutations in the MMP2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids. MMP-2 deficient mice exhibit slightly delayed growth, reduced neovascularization, retarded tumor progression, an exaggerated asthma response to allergens, and impaired branching morphogenesis of the mammary gland.
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分子量
- This 663 amino acid recombinant protein has a predicted molecular mass of approximately 74.5 kDa. The protein migrates at about 72 kDa in DTT-reducing conditions and about 71 kDa in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid i
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途径
- Activation of Innate immune Response
抗原
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