BMP4 Protein (AA 303-408)
Quick Overview for BMP4 Protein (AA 303-408) (ABIN2666447)
抗原
See all BMP4 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 303-408
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内毒素水平
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Less than 0.1 ng per μg of protein.
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Bone Morphogenetic Protein 4 (BMP4) (AA 303-408) protein (His-IF2DI Tag)
BMP4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Bone Morphogenetic Protein 4 (BMP4) (AA 293-408) proteinBMP4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: BMP-4 induces alkaline phosphatase production on ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml, corresponding to 1-2 x 105 unit/mg.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
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别名
- BMP-4
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背景
- Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGFbeta) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II / IIB activin receptors. BMP-4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish. BMP-2 and BMP-4 are highly similar, they have 92 % amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 KO mice are embryonic-lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP). Overexpression of BMP-4 has also been associated with cancer, including melanoma, hepatocellular carcinoma, adenocarcinoma and colorectal cancer cell lines. This overexpression can promote migratory and invasive properties of cancer cells.
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分子量
- The 106 amino acid recombinant protein has a predicted molecular mass of approximately 12 kDa. The DTT-non reduced protein migrates at approximately 31-36 kDa on SDS-PAGE. The predicted N-terminal amino acid is Lys.
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途径
- Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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