BMP4 Protein (AA 303-408)
-
- 抗原 See all BMP4 蛋白
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 303-408
-
宿主
- 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 纯度
- > 97 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.
- 内毒素水平
-
Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product BMP4 蛋白
-
Bone Morphogenetic Protein 4 (BMP4) (AA 303-408) protein (His-IF2DI Tag)
BMP4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Bone Morphogenetic Protein 4 (BMP4) (AA 293-408) proteinBMP4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Bone Morphogenetic Protein 4 (BMP4) (AA 25-408) protein (His-MBP)BMP4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func
Bone Morphogenetic Protein 4 (BMP4) (AA 293-408) protein (His tag)Crystallography grade BMP4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Bone Morphogenetic Protein 4 (BMP4) (AA 293-408) protein (His tag)Crystallography grade BMP4 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Biological activity: BMP-4 induces alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 5 - 15 ng/ml, corresponding to a specific activity of 0.67 - 2.0 x 105 units/mg.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute 10 mM citric acid to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
-
- 抗原
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
- 别名
- BMP-4 (BMP4 产品)
- 别名
- BMP2B Protein, BMP2B1 Protein, MCOPS6 Protein, OFC11 Protein, ZYME Protein, Bmp-4 Protein, Bmp2b Protein, Bmp2b-1 Protein, Bmp2b1 Protein, BOMPR4A Protein, bmp-4 Protein, zbmp-4 Protein, zgc:100779 Protein, BMP-4 Protein, XBMP-4 Protein, bmp2b Protein, bmp2b1 Protein, bmp4 Protein, ofc11 Protein, xbmp4 Protein, zyme Protein, BMP4 Protein, bone morphogenetic protein 4 Protein, bone morphogenetic protein 4 L homeolog Protein, bone morphogenetic protein 4 S homeolog Protein, BMP4 Protein, Bmp4 Protein, bmp4 Protein, bmp4.L Protein, bmp4.S Protein
- 背景
- Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Three type I receptors have been identified: type IA and IB BMP receptors and type IA activin receptors. Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. BMP-4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish. BMP-2 and BMP-4 are highly similar, they have 92 % amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 KO mice are embryonic lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression of BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP).
- 分子量
- The 106 amino acid recombinant protein has a predicted molecular mass of approximately 12 kDa. Recombinant BMP-4 is a disulphide bond linked homodimer. The predicted N-terminal amino acid is Lys.
- 途径
- Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
-
