CXCL13 Protein (AA 22-109)
Quick Overview for CXCL13 Protein (AA 22-109) (ABIN2666443)
抗原
See all CXCL13 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 22-109
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 22-109) protein (Fc Tag)
CXCL13 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 22-109) protein (His tag)CXCL13 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) protein (Fc Tag)CXCL13 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) protein (His-SUMO Tag)CXCL13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 1-109) protein (GST tag)CXCL13 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) protein (His tag)CXCL13 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 13 (CXCL13) (AA 23-109) protein (GST tag)CXCL13 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract BaF3-mCXCR5 transfectants in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in buffer containing carrier protein such as 1 % BSA or HSA or 10 % FBS. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C for six months.
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- CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))
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别名
- CXCL13
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背景
- CXCL13 was initially described as a chemokine, highly expressed in the follicles of Peyer's patches, spleen, and lymph nodes, and as a strong chomoattractant for B cells. CXCL13 is expressed by T follicular helper cells, dendritic cells, and stromal cells (including marginal reticular cells (MRCs) and follicular dendritic cells (FDCs)) in B cell areas of secondary lymphoid tissue. CXCL13 binds to CXCR5 that was initially cloned from Burkitt's lymphoma cells and named BLR1. B cells migrate in lymphoid organs in search of specific antigens. B cells enter lymph nodes through high endothelial venules and migrate through the lymph node follicle in a CXCL13/CXCR5 dependent manner. During the B cell migration in the follicle, they are in contact with FDCs, MRCs, and cells localized around the follicle perimeter, such as sinus-associated macrophages and T cell zone-associated DCs. Subsequent to the migration though the FDC network, the B cells travel though the T cell zone-proximal follicle. In this migration, CCL21, CCL19, CCR7 and CXCR5 are involved. CXCL13, CCL19, and CCL21 take part in the formation of tertiary lymphoid tissues in chronic antigen-induced arthritis. High expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, rheumatoid arthritis, and the allergic airway inflammatory process. In summary, CXCL13 is highly expressed at sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions.
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分子量
- The 78 amino acid recombinant protein has a predicted molecular mass of approximately 9.79 kDa. The DTT-reduced and non-reduced protein migrate at approximately 15 and 13 kDa by SDS-PAGE respectively. The N-terminal amino acid is Ile.
抗原
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